Egg culture techniques


Staff member
Sep 4, 2006
In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexes
D.B. Reis, N.G. Acosta, E. Almansa, J.C. Navarro, D.R. Tocher, O. Monroig, J.P. Andrade, A.V. Sykes, C. Rodríguez 2014 (subscription)

The high mortalities observed during Octopus vulgaris paralarvae culture have been associated with a nutritional imbalance, with long-chain polyunsaturated fatty acids (LC-PUFA) appearing to have a critical role. In order to determine the in vivo capability of O. vulgaris hatchlings to incorporate and metabolise unsaturated fatty acids (FA), hatchlings were incubated in flat-bottom 6-well tissue culture plates at a density of 90 hatchlings/well in 10 mL of seawater (36 ‰). Incubations were performed with gentle stirring at 21 ºC for 6 h with 0.2 μCi (0.3 μM) of [1-14C]-labelled FA including 18:1n-9, 18:2n-6, 18:3n-3, 20:4n-6 (ARA), 20:5n-3 (EPA) or 22:6n-3 (DHA), which were added directly to the seawater as their potassium salts bound to bovine serum albumin (BSA). A control treatment without [1-14C]FA was also assessed. O. vulgaris hatchlings not only possessed the ability to incorporate FA bound to BSA, but also to esterify them into phospholipid, with marked specificity. [1-14C]DHA and [1-14C]C18 FA substrates were mainly esterified into phosphatidylcholine, while [1-14C]ARA and [1-14C]EPA were esterified into phosphatidylethanolamine. The majority of radioactivity from [1-14C]FA incorporated into hatchling total lipid was recovered as unmodified FA with elongation being the only metabolism detected. Of the FA investigated, [1-14C]ARA was the most efficiently incorporated into hatchling lipids, but it was also the least modified FA. The fact that no desaturation activity was recorded towards the FA tested in this experiment may indicate that the nutritional requirements of O. vulgaris hatchlings in terms of FA are highly specific and LC-PUFA must be considered essential dietary nutrients.
Oct 28, 2014
Hi my blue ring left me around 100 eggs in a cave and iv been warned about the dangers but I'm cloud up enough to take care. The eggs will be hatching within a week at a guess I'm completely in prepared for this but still I don't think not to try to keep them is an option. Am I correct in thinking that it best to use nursery tanks only shallow filled and I was thinking just to use a pump and air stone for movement/oxygen and stock the tank water with brine shrim copypods and any other tiny bit of food I can. Iv been informed this has never been successfully done but still want to go ahead. Any extra help appreciated.
Sep 16, 2005
I'm definitely not a blue ring expert, but I do know there have been some cases where someone had contact with water in which a blue ring had died and then had a reaction. If you are doing water changes, you need to be extremely careful to protect yourself from contact with the water.

Well, it appears you only have to worry if they are dead. See this thread.
Last edited:

Latest Posts