Nautilus at the Steinhart Aquarium

[Thales]

Just got word that 9 new Nauts are headed to the Steinhart on Monday 10/23. Very excited. We are going to draw hemolymph on all of them and when the results combe back clear, they go on show.

 

[skywindsurfer]

What is hemolymph, and what are you looking for,..disease?

 

[esquid]

how much do you draw from them? I was wondering if you had enough to make some aliquots for me so I could do hemocyte counts from them.

erin

 

[Thales]

Erin, email me what you would need and why and I'll send it along to our vet who makes those kind of decisions.

[email protected]

 

[Thales]

What is hemolymph, and what are you looking for,..disease?

That is, the circulatory fluid is not returned to the heart through vessels, but instead passes through body spaces (sinuses) surrounding tissue as does our own lymph. Such fluid, instead of being called blood, is given the name hemolymph

and yep!

 

[esquid]

Will do. I had been thinking about nautilus hemocytes since talking to you and Greg about sampling the hemolymph. I looked and haven't been able to find anything published. I was figuring if I could get samples from people when they draw hemolymph for other purposes I could start compiling some information. They would have to be fixed to be shipped so I can do counts, but not any activity assays.

 

[DWhatley]

Where and how would you draw hemolymph? I am not looking for a detailed method but a general concept of how you would draw fluids that are not blood.

 

[Thales]

From a hemocoe, a pool of fluid.

l

 

[DWhatley]

but not from an organ like a kidney, stomach, etc right? If you could collect it, would urine provide the same type information?

 

[Thales]

Nope. A hemocoel is cavity or series of spaces between the organs. I would suspect that there would not be enough volume to collect from an organ, and I would be terrified to pierce a healthy organ to damage it.

 

[gjbarord]

That technique looks very familiar Rich.... I am so glad it is working out for y'all!! Any data come back yet?

Greg

 

[Thales]

We actually just did the physicals on Wednesday - things sometimes move slow at work (really? A box of cuttlebones still under my desk?). Ended up with only three animals - things sometimes don't arrive the way suppliers say they will.

Did everything you asked Greg. We are thinking of writing it up for Drum and Croaker as a follow up to your last write up. 2% on all of them, and they all ate the day after. Three females to go with my one male. Happy male.

 

[DWhatley]

Nauts are my second favorite cephs so I wish you would report on them more often

 

[Thales]

I wish I would to. Lots of crazy at work the last several months, and trying to get a handle on how to not go simply insane has been taking much of my time. Moving the new nauts onto exhibit will help, and I feel like I am starting to see light at the end of many tunnels. I hope!

 

[Thales]

how much do you draw from them? I was wondering if you had enough to make some aliquots for me so I could do hemocyte counts from them.

erin

Erin - the consensus is to look at this again after a few months. The amount drawn was very small. I'll email you on Monday.