Thawing Jumbo Squid for Research

Joined
Feb 18, 2007
Messages
76
I have three Humboldt squid that I need to defrost for my fourth year project. I will be doing stable isotope and DNA analysis and I am going to try testing for enzymes but since it was not kept at -80 I'm not sure if that will work. Each one weights about 15 pounds, maybe a bit more and they are about two and a half feet long (with the tentacles wrapped under them). What is the safest and fastest way to defrost them? Can I put them in a container full of cold water? About how long would it take for it to thaw?

Any help would be greatly appreciated!
 

OB

Colossal Squid
Staff member
Moderator
Joined
Oct 19, 2003
Messages
3,101
At a starting temp of minus 18 degrees Celcius and immersed in cold water it should not take a lot more than three hours, max. Isotonic water?
 

Steve O'Shea

TONMO Supporter
Supporter
Joined
Nov 19, 2002
Messages
4,671
Just cut a bit of flesh off first, if you're to do the DNA; not sure what enzymes you're looking for, or what defrosting will do to them. I'm with Ob - just place in cold water, and whatever you do, don't try and prise the animal apart when it is only partially defrosted - you'll just snap a limb or three if you do.
 

Steve O'Shea

TONMO Supporter
Supporter
Joined
Nov 19, 2002
Messages
4,671
I'm particularly interested in the beaks of this thing; PLEASE don't discard! (I'm mad keen on a set, if possible)
 
Joined
Feb 18, 2007
Messages
76
The good news is the thaw went very well! We were able to dissect and identify all the parts and get the tissue I need. I have another one to thaw this week, and this time I'll take lots of pictures!

The bad news is I forgot to throw out the bag of squidy water that we thawed her in and left for the weekend, and now the entire lab (and anyone who stands in it for too long) smells of bad squid...

I have the beak in the freezer for you, Steve, how would you like it preserved? Ethanol or dried out?
 

DWhatley

Kraken
Staff member
Moderator
Joined
Sep 4, 2006
Messages
20,981
I guess you caught Mola Mola disease from Steve but it has been dormant for a few years :roll:
 

OB

Colossal Squid
Staff member
Moderator
Joined
Oct 19, 2003
Messages
3,101
The Andromola strain.... Out of curiosity, how long did the thawing of the first squid last in total? And did you do total weight?
 

Top